Behavioural analysis of 6-hydroxydopamine rodent models of Parkinson’s disease

Background: Parkinson’s disease (PD) is most commonly modelled in rats via injection of the neurotoxin 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle. Assessment of lateralised choice reaction time is usually conducted in the 9 hole box, a highly specialized apparatus that is not widely available in many behavioural laboratories. The retractable lever Skinner box on the other hand is more readily available and may have the additional advantage of faster training of animals. Objective: The present study assesses the performance of lesioned rats on a lateralised choice reaction time task that allows for quantitative assessment of the behavioural profile of models of neurodegenerative diseases in the retractable lever Skinner box. Methods: Here we compared the post lesion performance of pretrained female Lister hooded rats that received unilateral 6OHDA lesions to the medial forebrain bundle and untreated controls on the choice reaction time task conducted in the Skinner box. Results: Lesioned animals displayed impairments in contralateral accuracy, a reduced number of usable trials, as well as a slowing down of contralateral reaction and bilateral movement time latencies. Conclusions: The findings presented allow greater comparison between laboratories, and may be useful for the investigation of treatment strategies and remedies on this model of PD.